Oomycete mtDNA: Phytophthora Genes for Cytochrome c Oxidase Use an Unmodified Genetic Code and Encode Proteins Most Similar to Those of Plants

Sachay, D. J., Hudspeth, D. S. S., Nadler, S. A., and Hudspeth, M. E. S. 1993. Oomycete mtDNA: Phytophthora genes for cytochrome c oxidase use an unmodified genetic code and encode proteins most similar to those of plants. Experimental Mycology 17, 7-23. A 3.6-kb region of mtDNA containing the genes...

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Published inExperimental mycology Vol. 17; no. 1; pp. 7 - 23
Main Authors Sachay, Donna J, Hudspeth, Deborah S.S, Nadler, Steven A, Hudspeth, Michael E.S
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.03.1993
Academic Press
Subjects
Biological and medical sciences
cytochrome c oxidase
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
oomycete protein
Oomycetes
Phytophthora
Phytophthora megasperma
oomycete protein
cytochrome c oxidase
Phytophthora megasperma
Fungi
Cytochrome c oxidase
Gene
Nucleotide sequence
Enzyme
Phycomycetes
Restriction map
Gene organization
Mitochondrial DNA
Thallophyta
Open reading frame
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Summary:Sachay, D. J., Hudspeth, D. S. S., Nadler, S. A., and Hudspeth, M. E. S. 1993. Oomycete mtDNA: Phytophthora genes for cytochrome c oxidase use an unmodified genetic code and encode proteins most similar to those of plants. Experimental Mycology 17, 7-23. A 3.6-kb region of mtDNA containing the genes for cytochrome c oxidase subunits I and II ( cox1, cox2) in the oomycete phytopathogen Phytophthora megasperma has been cloned and sequenced. Three uninterrupted reading frames of 456, 774, and 1476 base pairs encode an unidentified 18-kDa polypeptide (ORF152) and subunits II (COII) and I (COI) of cytochrome c oxidase. Analysis of codons for 146 invariant/highly conserved residues present in cox1 and cox2 suggests the use of an unmodified and unedited genetic code in the mitochondrial translation system. Comparisons of COI-and COII-deduced amino acid identities with the equivalent polypeptides from all biological kingdoms demonstrate that the oomycete proteins differ significantly from those of other reported cytochrome c oxidase subunits, but have the highest identity with plants.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0147-5975
1878-4399
DOI:10.1006/emyc.1993.1002