Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin

• Published in: Monoclonal antibodies in immunodiagnosis and immunotherapy Vol. 37; no. 5; p. 233
• Main Authors: Furusawa, Yoshikazu, Yamada, Shinji, Itai, Shunsuke, Sano, Masato, Nakamura, Takuro, Yanaka, Miyuki, Handa, Saori, Mizuno, Takuya, Maeda, Ken, Fukui, Masato, Harada, Hiroyuki, Kaneko, Mika K, Kato, Yukinari
• Format: Journal Article
• Language: English
• Published: United States 01.11.2018
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibody Specificity - immunology; CHO Cells; Cricetinae; Cricetulus; Epitope Mapping; Epitopes - immunology; Flow Cytometry; Horses - immunology; Humans; Lectins, C-Type - immunology; Membrane Glycoproteins - genetics; Membrane Glycoproteins - immunology; Mice; Platelet Aggregation - immunology; Podocytes - immunology; horse podoplanin; PDPN; PMab-202
• ISSN: 2167-9436
• DOI: 10.1089/mab.2018.0030

Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs. One of the established mAbs, PMab-202 (IgG , kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN.