Reactive oxygen species mediate lethality induced by far-UV in Escherichia coli cells

The involvement of reactive oxygen species (ROS) in the induction of DNA damage to Escherichia coli cells caused by UVC (254 nm) irradiation was studied. We verified the expression of the soxS gene induced by UVC (254 nm) and its inhibition by sodium azide, a singlet oxygen ( 1 O 2 ) scavenger. Addi...

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Bibliographic Details
Published inRedox report : communications in free radical research Vol. 10; no. 2; pp. 91 - 95
Main Authors Gomes, A.A., Silva-Júnior, A.C.T., Oliveira, E.B., Asad, L.M.B.O., Reis, N.C.S.C., Felzenszwalb, I., Kovary, K., Asad, N.R.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.04.2005
Subjects
Bacterial Physiological Phenomena
Bacterial Proteins - chemistry
Carotenoids - chemistry
Carotenoids - pharmacology
Dose-Response Relationship, Radiation
ESCHERICHIA COLI
Escherichia coli - metabolism
Escherichia coli - radiation effects
Escherichia coli Proteins - chemistry
Free Radical Scavengers - chemistry
Free Radicals
NORBIXIN
Oxygen - chemistry
Reactive Oxygen Species
Sodium Azide - chemistry
Sodium Azide - pharmacology
SOXRS REGULON
Trans-Activators - chemistry
Transcription Factors - chemistry
Ultraviolet Rays
UVC
Water - chemistry
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Summary:The involvement of reactive oxygen species (ROS) in the induction of DNA damage to Escherichia coli cells caused by UVC (254 nm) irradiation was studied. We verified the expression of the soxS gene induced by UVC (254 nm) and its inhibition by sodium azide, a singlet oxygen ( 1 O 2 ) scavenger. Additional results showed that a water-soluble carotenoid (norbixin) protects against the lethal effects of UVC. These results suggest that UVC radiation can also cause ROS-mediated lethality.
ISSN:1351-0002
1743-2928
DOI:10.1179/135100005X38833