Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with go...

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Published inMonoclonal antibodies in immunodiagnosis and immunotherapy Vol. 38; no. 2; p. 96
Main Authors Yamada, Shinji, Kaneko, Mika K, Furusawa, Yoshikazu, Itai, Shunsuke, Sano, Masato, Nakamura, Takuro, Yanaka, Miyuki, Handa, Saori, Hisamatsu, Kayo, Nakamura, Yoshimi, Koyanagi, Masanori, Fukui, Masato, Harada, Hiroyuki, Kato, Yukinari
Format Journal Article
LanguageEnglish
Published United States 01.04.2019
Subjects
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity - immunology
Cattle
CHO Cells
Cricetinae
Cricetulus
Goats
Immunohistochemistry - methods
Lung - immunology
Lung - metabolism
Membrane Glycoproteins - immunology
PMab-44
PDPN
goat podoplanin
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Summary:Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG , kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.
ISSN:2167-9436
DOI:10.1089/mab.2018.0031