Insights into the Cardioprotective Effects of Pyridoxine Treatment in Diabetic Rats: A Study on Cardiac Oxidative Stress, Cardiometabolic Status, and Cardiovascular Biomarkers

• Published in: Diagnostics (Basel) Vol. 14; no. 14; p. 1507
• Main Authors: Mutavdzin Krneta, Slavica, Gopcevic, Kristina, Stankovic, Sanja, Jakovljevic Uzelac, Jovana, Todorovic, Dušan, Labudovic Borovic, Milica, Rakocevic, Jelena, Djuric, Dragan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2024
• Subjects: Anticoagulants; Antioxidants; Atherosclerosis; Biomarkers; cardiac oxidative stress; Cardiomyocytes; Cardiovascular disease; Cholesterol; Dehydrogenases; Diabetes; diabetes mellitus; Enzymes; Ethylenediaminetetraacetic acid; Glucose; Heart; Laboratory animals; Metabolism; Oxidation; Oxidative stress; Plasma; pyridoxine; rat; Streptozocin; streptozotocin; Superoxide; Vitamin B; Serbia; United States > US; Germany; diabetes mellitus; pyridoxine; rat; streptozotocin; cardiac oxidative stress
• ISSN: 2075-4418; 2075-4418
• DOI: 10.3390/diagnostics14141507

The aims of this study were to examine the effects of pyridoxine administration on the activities of cardiac antioxidant stress enzymes superoxide dismutase (SOD) and catalase (CAT) and enzyme indicators of cardiometabolic status, lactate and malate dehydrogenase (LDH, MDH), as well as LDH and MDH isoforms' distribution in the cardiac tissue of healthy and diabetic Wistar male rats. Experimental animals were divided into five groups: C1-control (0.9% sodium chloride-NaCl-1 mL/kg, intraperitoneally (i.p.), 1 day); C2-second control (0.9% NaCl 1 mL/kg, i.p., 28 days); DM-diabetes mellitus (streptozotocin 100 mg/kg in 0.9% NaCl, i.p., 1 day); P-pyridoxine (7 mg/kg, i.p., 28 days); and DM + P-diabetes mellitus and pyridoxine (streptozotocin 100 mg/kg, i.p., 1 day and pyridoxine 7 mg/kg, i.p., 28 days). Pyridoxine treatment reduced CAT and MDH activity in diabetic rats. In diabetic rats, the administration of pyridoxine increased LDH1 and decreased LDH4 isoform activities, as well as decreased peroxisomal MDH and increased mitochondrial MDH activities. Our findings highlight the positive effects of pyridoxine administration on the complex interplay between oxidative stress, antioxidant enzymes, and metabolic changes in diabetic cardiomyopathy.