Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle

• Published in: Journal of visualized experiments no. 136
• Main Authors: Cano Garcia, Maria, Nesbit, Steven C, Le, Chi C, Dearworth, Jr, James R
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 02.06.2018
• Subjects: Animals; Biomechanical Phenomena; Cranial Nerves - physiology; Electric Stimulation - methods; Eye Movements - physiology; Neuroscience; Turtles; Vision, Ocular - physiology
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/56864

After animals are euthanized, their tissues begin to die. Turtles offer an advantage because of a longer survival time of their tissues, especially when compared to warm-blooded vertebrates. Because of this, in vitro experiments in turtles can be performed for extended periods of time to investigate the neural signals and control of their target actions. Using an isolated head preparation, we measured the kinematics of eye movements in turtles, and their modulation by electrical signals carried by cranial nerves. After the brain was removed from the skull, leaving the cranial nerves intact, the dissected head was placed in a gimbal to calibrate eye movements. Glass electrodes were attached to cranial nerves (oculomotor, trochlear, and abducens) and stimulated with currents to evoke eye movements. We monitored eye movements with an infrared video tracking system and quantified rotations of the eyes. Current pulses with a range of amplitudes, frequencies, and train durations were used to observe effects on responses. Because the preparation is separated from the brain, the efferent pathway going to muscle targets can be examined in isolation to investigate neural signaling in the absence of centrally processed sensory information.