The Degradation Effects of a Pseudomonas Hydrolase OPHC2 to Organophosphorus Insecticides

The degradation effects of OPHC2 to 9 organophosphates and other pesticides were studied. The degrading reaction was carried out for 10 minutes under 37 °C in 50 mmol pH 8.0 Tris-HCl buffer. Organophosphates and sulfonylurea herbicides were detected by HPLC-UV and/or HPLC/MS. It's shown that de...

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Published inPhosphorus, sulfur, and silicon and the related elements Vol. 183; no. 2-3; pp. 804 - 810
Main Authors Wang, Jin-Fang, Gao, Ming-Hui, Wu, Ning-Feng, Pan, Can-Ping
Format Journal Article
LanguageEnglish
Published Taylor & Francis Group 01.02.2008
Subjects
Enzymatic degradation
OPHC2
organophosphates
Pseudomonas hydrolase
pyrethroids
sulfonylurea herbicides
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Summary:The degradation effects of OPHC2 to 9 organophosphates and other pesticides were studied. The degrading reaction was carried out for 10 minutes under 37 °C in 50 mmol pH 8.0 Tris-HCl buffer. Organophosphates and sulfonylurea herbicides were detected by HPLC-UV and/or HPLC/MS. It's shown that degradation rates of 70%-100% were achieved for most of the organophosphates after 24 h (chlorpyrifos 100%, phoxim 100%, fenitrothion 99.87%, isocarbophos 100%, dimethoate 78.55%, methamidophos 71.55%, methidathion 87.93%). Pyrethroids were detected by GC-ECD after extracted by hexane. The degrading amounts of 5 pyrethroids were all above 50% after 3 hours (bifenthrin 78.7%, lambda-cyhalothrin 84.6%, permethrin 69.1%, beta-cypermethrin 51.5%, fenvalerate 72.2%). What is interesting, the degrading amount of malathion in its single standard solution was 94.31% while it did not degrade at all in the mixture standard even under superfluous enzyme solution. OPHC2 showed no effect to the sulfonylurea herbicides.
ISSN:1042-6507
1563-5325
DOI:10.1080/10426500701808234