Passenger vehicle interior decontamination by low concentration hydrogen peroxide vapor following a wide area biological contamination incident
To assess low concentration hydrogen peroxide (LCHP) (H2O2) vapor dispersed with a home humidifier for its ability to decontaminate vehicle interiors contaminated with Bacillus anthracis surrogate Bacillus atrophaeus spores. Efficacy of a vaporized 3% H2O2 solution was evaluated for liquid volumes,...
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Published in | Journal of applied microbiology Vol. 134; no. 3 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.03.2023
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Subjects | |
Online Access | Get full text |
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Summary: | To assess low concentration hydrogen peroxide (LCHP) (H2O2) vapor dispersed with a home humidifier for its ability to decontaminate vehicle interiors contaminated with Bacillus anthracis surrogate Bacillus atrophaeus spores.
Efficacy of a vaporized 3% H2O2 solution was evaluated for liquid volumes, on/off vehicle heating, ventilation, and air conditioning (HVAC) system operations, and temperatures that ranged from 5 to 27°C. Survival of the spores was assessed by quantification of remaining viable spores with efficacy quantified in terms of mean log10 reduction. Decontamination efficacy after the 6-day dwell time increased when the 3% H2O2 liquid volume was doubled, increasing from 4-of-10 to 10-of-10 nondetects (zero colonies counted using standard dilution and filter plating) inside the vehicle cabin. Recirculating cabin air through the HVAC system during decontamination decreased efficacy to 6-of-10 non-detects. While no 6-log10 reduction in viable spores was observed on the cabin filter with the cabin filter kept in place, a 6-log10 reduction was achieved after its removal and placement in the cabin during treatment.
Results from this study allow for informed decisions on the use of LCHP vapor as an effective decontamination approach for vehicle interiors. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Lukas Oudejans (Conceptualization, Investigation, Methodology, Project administration, Writing – original draft), William Richter (Conceptualization, Formal analysis, Investigation, Methodology, Supervision, Writing – original draft), Michelle Sunderman (Formal analysis, Investigation, Visualization, Writing – review & editing ), M. Worth Calfee (Conceptualization, Investigation, Writing – review & editing), R. Leroy Mickelsen (Conceptualization, Investigation, Writing – review & editing), Kent Hofacre (Investigation, Methodology, Project administration, Writing – review & editing ), Patrick Keyes (Investigation, Methodology), and Sang Don Lee (Conceptualization, Writing – review & editing) Author Contributions |
ISSN: | 1365-2672 1364-5072 1365-2672 |
DOI: | 10.1093/jambio/lxad039 |