Effect of Lithothamnium sp and calcium supplements in strain- and infection-induced bone resorption

• Published in: The Angle orthodontist Vol. 84; no. 6; pp. 980 - 988
• Main Authors: de Albuquerque Taddei, Silvana Rodrigues, Madeira, Mila Fernandes Moreira, de Abreu Lima, Izabella Lucas, Queiroz-Junior, Celso Martins, Moura, Adriana Pedrosa, Oliveira, Dauro Douglas, Andrade, Jr, Ildeu, da Glória Souza, Danielle, Teixeira, Mauro Martins, da Silva, Tarcília Aparecida
• Format: Journal Article
• Language: English
• Published: United States Edward H Angle Education and Research Foundation, Inc 01.11.2014
• Subjects: Acid Phosphatase - analysis; Aggregatibacter actinomycetemcomitans - physiology; Alveolar Bone Loss - blood; Alveolar Bone Loss - microbiology; Alveolar Bone Loss - prevention & control; Alveolar Process - drug effects; Animals; Bone Density Conservation Agents - therapeutic use; Calcitriol - blood; Calcium - blood; Calcium Carbonate - therapeutic use; Calcium, Dietary - therapeutic use; Cell Count; Dietary Supplements; Disease Models, Animal; Isoenzymes - analysis; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Original; Osteoclasts - drug effects; Osteoclasts - pathology; Pasteurellaceae Infections - microbiology; Periodontal Diseases - microbiology; Rhodophyta - chemistry; Tartrate-Resistant Acid Phosphatase; Tooth Movement Techniques - adverse effects; Tooth Movement Techniques - instrumentation; Tumor Necrosis Factor-alpha - analysis; Bone resorption; Calcium; Orthodontic tooth movement
• ISSN: 0003-3219; 1945-7103
• DOI: 10.2319/080313-579.1

To investigate the effect of Lithothamnium sp (LTT) supplement, a calcium-rich alga widely used for mineral reposition, on strain-induced (orthodontic tooth movement [OTM]) and infection-induced bone resorption (periodontal disease [PD]) in mice. Mice were divided into two bone resorption models: one with an orthodontic appliance and the other with PD induced by the oral inoculation of Aggregatibacter actinomycetencomitans (Aa). Both groups were fed a regular diet (vehicle), LTT-rich diet (LTT), or calcium-rich diet (CaCO3). Alveolar bone resorption (ABR), the number of osteoclasts, and the levels of tumor necrosis factor α (TNF-α), calcium, and vitamin D3 were evaluated. The number of osteoclasts was reduced in LTT and CaCO3 mice, which led to diminished OTM and infection-induced alveolar bone loss. In addition, LTT- and calcium-treated groups also presented decreased levels of TNF-α in periodontal tissues and increased levels of calcium in serum. These results indicate that the LTT supplement influences ABR, probably due to its calcium content, by affecting osteoclast function and local inflammatory response, thus modulating OTM and PD.