Lentzea sokolovensis sp. nov., Lentzea kristufekii sp. nov. and Lentzea miocenica sp. nov., rare actinobacteria from Miocene lacustrine sediment of the Sokolov Coal Basin, Czech Republic

• Published in: International journal of systematic and evolutionary microbiology Vol. 74; no. 4
• Main Authors: Lara, Ana Catalina, Kotrbová, Lucie, Keller, Moritz, Nouioui, Imen, Neumann-Schaal, Meina, Mast, Yvonne, Chroňáková, Alica
• Format: Journal Article
• Language: English
• Published: England 01.04.2024
• Subjects: Actinobacteria; Actinomycetales; Bacteria; Bacterial Typing Techniques; Base Composition; Coal; Czech Republic; DNA, Bacterial - genetics; Fatty Acids - chemistry; Phosphatidylethanolamines; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Czech Republic; Lentzea; Actinomycetes; post-mining sites; polyphasic taxonomy
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.006335

The taxonomic position of three actinobacterial strains, BCCO 10_0061 , BCCO 10_0798 , and BCCO 10_0856 , recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061 in the same cluster as , strain BCCO 10_0798 in the same cluster as , , , and , and strain BCCO 10_0856 clustered together with and . Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus . In all three strains, MK-9(H ) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C , anteiso-C , iso-C , and C . The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061 , 69.2 for BCCO 10_0798 , and 68.5 for BCCO 10_0856 . The combination of digital DNA-DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061 , BCCO 10_0798 , and BCCO 10_0856 distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly-l-lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus , for which we propose the name sp. nov. for BCCO 10_0061 (=DSM 116175 ), sp. nov. for BCCO 10_0798 (=DSM 116176 ), and sp. nov. for BCCO 10_0856 (=DSM 116177 ).