Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52

• Published in: Monoclonal antibodies in immunodiagnosis and immunotherapy Vol. 37; no. 5; p. 224
• Main Authors: Yamada, Shinji, Itai, Shunsuke, Furusawa, Yoshikazu, Sano, Masato, Nakamura, Takuro, Yanaka, Miyuki, Handa, Saori, Hisamatsu, Kayo, Nakamura, Yoshimi, Fukui, Masato, Harada, Hiroyuki, Mizuno, Takuya, Sakai, Yusuke, Ogasawara, Satoshi, Murata, Takeshi, Uchida, Hiroaki, Tahara, Hideaki, Kaneko, Mika K, Kato, Yukinari
• Format: Journal Article
• Language: English
• Published: United States 01.11.2018
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity - immunology; Cats; Cattle; CHO Cells; Cricetulus; Epitope Mapping; Epitopes - immunology; Flow Cytometry; Humans; Membrane Glycoproteins - immunology; Mice; Podocytes - immunology; Rabbits; Rats; Tigers - immunology; tiger podoplanin; PDPN; PMab-52
• ISSN: 2167-9436
• DOI: 10.1089/mab.2018.0033

Podoplanin (PDPN) is expressed in type I alveolar cells of lung but not in type II alveolar cells. PDPN is also known as a specific lymphatic endothelial cell marker because PDPN is not expressed in vascular endothelial cells. PDPNs of several animals have been characterized using specific anti-PDPN monoclonal antibodies (mAbs): PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 for mouse, rat, rabbit, dog, bovine, and cat PDPNs, respectively. In this study, we investigated the possible crossreaction between these anti-PDPN mAbs and tiger PDPN. Flow cytometry and western blot analyses revealed that the anti-cat PDPN mAb PMab-52 (IgM, kappa) reacted with tiger PDPN, which is overexpressed in Chinese hamster ovary-K1 cells. Using immunohistochemical analysis, type I alveolar cells of the tiger lung were strongly detected by PMab-52. These results indicate that PMab-52 may be useful for the detection of tiger PDPN.