5,10,15,20-Tetrakis (4-carboxyphenyl) porphin-conjugated poly(l-lactic) acid/polyethylene oxide nanofiber membranes for photodynamic therapy

• Published in: Journal of photochemistry and photobiology. A, Chemistry. Vol. 355; pp. 267 - 273
• Main Authors: Ma, Fang, Yuan, Chun-Wang, Ren, Xiao-Xue, You, Chang-Jiang, Cao, Jian-Hua, Wu, Da-Yong
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.03.2018; Elsevier BV
• Subjects: Cancer; Chemical bonds; Confocal microscopy; Contact angle; Controlled release; Cytotoxicity; Drug carrier; Electron paramagnetic resonance; Electron spin; Electron spin resonance; Electrospun nanofiber; Fourier transforms; HeLa cells; Infrared spectroscopy; Irradiation; Light irradiation; Membranes; Nanofibers; Organic chemistry; Photodynamic therapy; Polyethylene; Polyethylene oxide; Polyethylenes; Polylactic acid; Scanning electron microscopy; Scanning microscopy; Singlet oxygen; Spectroscopy; Spin resonance; Sustained release; Tumor cells; HeLa cells; Electrospun nanofiber; Drug carrier; Singlet oxygen; Photodynamic therapy
• ISSN: 1010-6030; 1873-2666
• DOI: 10.1016/j.jphotochem.2017.08.062

[Display omitted] •We prepared a PLLA/PEO nanofiber membrane containing TCPP to treat cancer cells.•PEO improved the hydrophilicity of the nanofibers.•TCPP produced 1O2 at light irradiation (532nm).•The nanofiber membranes were cytotoxic in cancer cells under light irradiation. In this work, a poly(l-lactic) acid (PLLA)/polyethylene oxide (PEO) nanofiber membrane was prepared by electrospinning as a carrier for the photosensitizer 5,10,15,20-tetrakis (4-carboxyphenyl) porphin (TCPP) to study the effects of singlet oxygen (1O2) on tumor cells. Scanning electron microscopy and confocal laser scanning microscopy images showed that the fiber surface was smooth and that TCPP was evenly dispersed in the nanofibers. TCPP, PLLA, and PEO were mixed with nanofibers without the formation of new chemical bonds, as shown by Fourier transform infrared spectroscopy. The addition of PEO improved the hydrophilicity of the nanofibers, making the contact angle of the PLLA nanofiber membrane change from 93.3° to near 0° and resulting in good cell compatibility. The sustained release experiment showed that when the amount of TCPP was 3%, the cumulative release concentration after 72h was close to saturation in the test system. Electron spin resonance spectroscopy confirmed that TCPP produced 1O2 at light irradiation (532nm). The results of in vitro cell experiments showed that TCPP@PLLA/PEO nanofiber membranes did not affect the normal growth of HeLa cells but had cytotoxic effects on cancer cells under light irradiation.