Dual positional specificity of wound-responsive lipoxygenase from maize seedlings

• Published in: Journal of plant physiology Vol. 159; no. 11; pp. 1263 - 1265
• Main Authors: Kim, Eun-Seon, Kim, Haeyoung, Park, Ro-Dong, Lee, Youngman, Han, Oksoo
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.11.2002; Elsevier; Elsevier Science Ltd
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Enzymes; Fundamental and applied biological sciences. Psychology; jasmonic acid; lipoxygenase; Metabolism; Nutrition. Photosynthesis. Respiration. Metabolism; Plant physiology and development; stress; wound; lipoxygenase; stress; wound; jasmonic acid; Monocotyledones; Zea mays; Enzyme; Phylogenetic tree; Injury; Biological evolution; Plant juvenile growth stage; Regiospecificity; Cereal crop; Gramineae; Angiospermae; Stereospecificity; Spermatophyta; Oxidoreductases; Lipoxygenase; Enzymatic reaction
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1078/0176-1617-00898

Wound-responsive lipoxygenase full-length cDNA from Zea mays was used to heterologously express the lipoxygenase enzyme and positional specificity of the lipoxygenase reaction was determined. The purified lipoxygenase catalyzed the conversion of α-linolenic acid into both 13-hydroperoxylinolenic acid and 9-hydroperoxylinolenic acid with a ratio of 6 to 4. The phylogenetic tree analysis indicated that the lipoxygenase is a type 1-lipoxygenase and belongs to 9-lipoxygenase subfamily with exceptional positional specificity. Dual positional specificity of the wound-responsive lipoxygenase indicates the versatile utilization of a singular lipoxygenase species as both 13-lipoxygenase and 9-lipoxygenase.