Epidemiology of resistance and phenotypic characterization of carbapenem resistance mechanisms in Klebsiella pneumoniae isolates at Sahloul University Hospital-Sousse, Tunisia

• Published in: African health sciences Vol. 19; no. 2; pp. 2008 - 2020
• Main Authors: Messaoudi, Aziza, Mansour, Wejdène, Jaidane, Nedia, Chaouch, Chrifa, Boujaâfar, Noureddine, Bouallègue, Olfa
• Format: Journal Article
• Language: English
• Published: Uganda Makerere Medical School 01.06.2019
• Subjects: Carbapenems - pharmacology; Drug Resistance, Bacterial; Humans; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - isolation & purification; Microbial Sensitivity Tests; Phenotype; Retrospective Studies; Tunisia - epidemiology; Tunisia; ESBL; epidemiology; phenotypic screening; carbapenemase; Klebsiella pneumoniae
• ISSN: 1680-6905; 1729-0503; 1680-6905
• DOI: 10.4314/ahs.v19i2.24

To assess the prevalence of ESBL producing and carbapenem resistant isolated from in-come and out-come patients at Sahloul-university hospital. A retrospective study over a 3 years period (January 2012 and December 2014) focused on 2160 strains of . Statistical analysis was carried out using SPSS program. ESBL detection was performed using a double disc diffusion method and carbapenemase detection was realized by Rosco-Disk kit. A total of 2160 strains were isolated during the period of the study, 26.2% (n=566) were ESBL-producers and 15.8% (n=342) showed resistance to carbapenem. The wards most affected by these strains were basically urology and intensive care units. Eighty four percent of studied strains (203/241) were resistant to temocillin, which correlate with the production of a class D (OXA-48-like) carbapenemase and 7% (17/241) showed sensitivity to EDTA and dipicolinic acid, which indicate the production of metallo-enzyme. The rate of resistance to colistin remains low. Resistance of , including , to third generation cephalosporins (3rd GC) and carbapenem through the mechanism of ESBL and carbapenemases production is becoming increasingly worrying. This suggests a more rational use of antibiotics, as well as the rigorous application of hygiene measurement.