PM10 impairs the antioxidant defense system and exacerbates oxidative stress driven cell death

The aim of this study was to investigate the effect of airborne particulate matter with a mean aerodynamic diameter of a[control][curren]10I14m (PM10) on oxidative stress markers and antioxidant enzymatic activity and its relevance in the face of acute oxidative challenge in a human lung epithelial...

Full description

Saved in:
Bibliographic Details
Published inToxicology letters Vol. 193; no. 3; pp. 209 - 216
Main Authors CHIRINO, Yolanda I, SANCHEZ-PEREZ, Yesennia, OSORNIO-VARGAS, Alvaro R, MORALES-BARCENAS, Rocío, CONCEPCION GUTIERREZ-RUIZ, Maria, SEGURA-GARCIA, Yazmin, ROSAS, Irma, PEDRAZA-CHAVERRI, José, GARCIA-CUELLAR, Claudia M
Format Journal Article
LanguageEnglish
Published Shannon Elsevier 01.04.2010
Subjects
Antioxidants
Biological and medical sciences
Catalase
Cell death
Damage
Enzymes
Exposure
Glutathione
Medical sciences
Stresses
Toxicology
Oxidative stress
Cell death
Aerosols
Organism defense
Antioxidant
PM
Apoptosis
Coarse particle
Antioxidant enzymatic defense
Online AccessGet full text

Cover

More Information
Summary:The aim of this study was to investigate the effect of airborne particulate matter with a mean aerodynamic diameter of a[control][curren]10I14m (PM10) on oxidative stress markers and antioxidant enzymatic activity and its relevance in the face of acute oxidative challenge in a human lung epithelial cell line (A549). PM10-induced reactive oxygen species (ROS) generation and oxidative damage with no changes in cellular viability. In addition, PM10 decreased glutathione (GSH) levels (54.9%) and the activity of the antioxidant enzymes superoxide dismutase (65%), catalase (31.2%), glutathione reductase (61.5%) and glutathione-S-transferase (42.39%). Trolox, a scavenger of reactive species, prevented the increase of ROS generation and the decrease in GSH levels but partially prevented PM10-induced oxidative damage. Interestingly, it was unable to avoid the decrease in the activity of antioxidant enzymes. Finally, the survival of the cells previously exposed to PM10 and challenged with hydrogen peroxide was significantly lower. We conclude that the impairment in the antioxidant defense system induced by PM10 weaken ROS detoxification which exacerbates cell death when these cells are exposed to an acute oxidative challenge.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-4274
1879-3169
DOI:10.1016/j.toxlet.2010.01.009