Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100

Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known onl...

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Bibliographic Details
Published inMolecular microbiology Vol. 4; no. 6; p. 1019
Main Authors Dempsey, W B, Fee, B E
Format Journal Article
LanguageEnglish
Published England 01.06.1990
Subjects
Bacterial Proteins - genetics
Base Sequence
Chloramphenicol Resistance
Chromosome Mapping
Conjugation, Genetic
Deoxyribonuclease I
DNA-Binding Proteins - genetics
Escherichia coli - genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial
Integration Host Factors
Molecular Sequence Data
Mutation
Plasmids
Promoter Regions, Genetic
Sequence Homology, Nucleic Acid
Transcription, Genetic
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Summary:Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation gels, is near the traJ promoter. The relative promoter activities of genes X, traJ and traM are reduced in himA mutants (IHF-), as measured by chloramphenicol-resistance assays. Transcript analyses by Northern blots showed a reduction in size of the principal gene X and traJ transcripts in the absence of IHF.
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1990.tb00674.x