Dynamic Narrowing of the Diaphragmatic Vena Cava in Ovis aries

• Published in: Anatomia, histologia, embryologia Vol. 53; no. 6; pp. e13114 - n/a
• Main Authors: Schwartzman, William E., Turner, Mackenzie E., Spiess, J. Logan, Jimenez, Michael, Watanabe, Tatsuya, Hama, Rikako, Che, Jingru, Kelly, Gracie L., Yimit, Asigul, Baker, Peter B., Arsuaga‐Zorrilla, Carmen, Kelly, John, Breuer, Christopher K., Best, Cameron A., Reinhardt, James W.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.11.2024
• Subjects: Animal models; Animals; cardiovascular research; central venous pressure; Central Venous Pressure - physiology; Diaphragm; Diaphragm - anatomy & histology; Dorset (sheep breed); Female; Gravity; haemodynamics; Hemodynamics; Hemodynamics - physiology; histology; inferior vena cava; Magnetic Resonance Imaging - veterinary; ovine models; Ovis aries; Physiology; positional variations; sheep; Sheep - anatomy & histology; Smooth muscle; species; Thorax; vena cava; Vena Cava, Inferior - anatomy & histology; Vena Cava, Inferior - diagnostic imaging; ovine models; vena cava; positional variations; central venous pressure; cardiovascular research; haemodynamics
• ISSN: 0340-2096; 1439-0264; 1439-0264
• DOI: 10.1111/ahe.13114

ABSTRACT Dorset sheep (Ovis aries) are common models in translational cardiovascular research due to physiologic and anatomic similarities to humans. While employing ovine subjects to study single‐ventricle physiology, we repeatedly observed position‐based changes in central venous pressure (CVP) which could not be explained by hydrostatic (gravitational) effects. Inferior vena cava (IVC) narrowing or compression has been demonstrated in numerous species, and we hypothesised that this phenomenon might explain our observations in O. aries. This study aimed to characterise position‐dependent morphology of the IVC in O. aries using catheter‐based hemodynamic and dimensional measurements, three‐dimensional MRI reconstruction and histological analysis. Baseline measurements revealed a significant reduction in IVC dimensions at the level of the diaphragm (dVC) compared to the abdominal vena cava (aVC) and thoracic vena cava (tVC). We also observed a transdiaphragmatic pressure gradient along the IVC, with higher pressures in the aVC compared to the tVC. We found that variation of position and fluid status altered IVC haemodynamics. Histological data showed variable muscularity along the length of the IVC, with greater smooth muscle content in the aVC than the tVC. These findings will improve understanding of baseline ovine physiology, help refine experimental protocols and facilitate the translation of findings to the clinic.