RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

• Published in: Applied biological chemistry Vol. 63; no. 1; p. 30
• Main Authors: Han, Ying-Hao, Mao, Ying-Ying, Yu, Nan-Nan, Jin, Mei-Hua, Jin, Ying-Hua, Wang, Ai-Guo, Zhang, Yong-Qing, Shen, Gui-Nan, Cui, Yu-Dong, Yu, Li-Yun, Lee, Dong-Seok, Jo, Yu-Jin, Sun, Hu-Nan, Kwon, Jeongwoo, Kwon, Taeho
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.12.2020; Springer Nature B.V; 한국응용생명화학회
• Subjects: Applied Microbiology; Biological Techniques; Bioorganic Chemistry; cell transplantation; Chemistry; Chemistry and Materials Science; Encyclopedias; Gene expression; gene expression regulation; gene ontology; Gene sequencing; gene targeting; Genes; genome; Genomes; Graft rejection; Mesenchymal stem cells; Rejection; Ribonucleic acid; RNA; sequence analysis; Stem cell transplantation; Stem cells; Survival; survival rate; Transplantation; 농학; RNA sequencing; Allograft rejection; Dermal mesenchymal stem cells; Peroxiredoxin II
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1186/s13765-020-00515-z

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p  ≤ 0.05) between the PrxII +/+ (WT) and PrxII −/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII −/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII −/− vs. PrxII +/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.