Phenolic and carotenoid characterization of the ethanol extract of an Australian native plant Haemodorum spicatum

• Published in: Food chemistry Vol. 399; p. 133969
• Main Authors: Liang, Zijian, Zhang, Pangzhen, Xiong, Yun, Johnson, Stuart K., Fang, Zhongxiang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15.01.2023
• Subjects: Antioxidant activity; Carotenoids; Haemodorum spicatum; HPLC-DAD; HPLC-DAD-ESI-QTOF-MS/MS; Phenolic compounds; Haemodorum spicatum; Carotenoids; HPLC-DAD; Phenolic compounds; Antioxidant activity; HPLC-DAD-ESI-QTOF-MS/MS
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.133969

•Bloodroot bulb extract had relatively low phenolic content and antioxidant activity.•UV maximum absorption wavelength of the bloodroot bulb extract was 477 nm.•Lutein and capsanthin were detected at relatively low levels in the bulb extract.•A total of 40 phenolic compounds were tentatively identified in the bulb extract. Bloodroot (Haemodorum spicatum) is an Australian native bulb plant yielding red pigment. This study aimed to characterize the phenolic and carotenoid profiles of the 80% ethanol extract of the H. spicatum bulb by HPLC-DAD-ESI-QTOF-MS/MS and HPLC-DAD. Results revealed the relatively low total phenolic content and antioxidant activity of the bulb extract with the maximum absorbance at 477 nm. Only 2 carotenoids (lutein and capsanthin) were detected at relatively low levels in the extract. A total of 40 phenolic compounds were tentatively identified, including 5 phenolic acids, 13 flavonoids and 22 other phenolic compounds, where 35 were reported for the first time in H. spicatum, together with 3 previously reported phenylphenalenones, haemodorol, haemoxiphidone and 2,5,6-trimethoxy-9-phenyl-1H-phenalen-1-one, and 2 oxabenzochrysenones, 5-hydroxy-2-methoxy-1H-naphtho[2,1,8-mna]xanthen-1-one and 5-hydroxy-1H-naphtho[2,1,8-mna]xanthen-1-one. This study provided the most comprehensive phenolic and carotenoid profiles of H. spicatum up to date.