Microbial community and function evaluation in the start-up period of bioaugmented SBR fed with aniline wastewater

• Published in: Bioresource technology Vol. 319; p. 124148
• Main Authors: Zhang, Wenli, Zhang, Qian, Li, Meng, Wang, Hongyu, Li, Yao, Peng, Haojin, Feng, Jiapeng
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.01.2021
• Subjects: Aniline; Bioaugmentation; Functional gene; Metabolic pathway; Microbial community; Metabolic pathway; Aniline; Bioaugmentation; Functional gene; Microbial community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2020.124148

[Display omitted] •A bioaugmented SBR with high-efficiency degradation of aniline was start-up.•Thauera contributed the most to aniline degradation and nitrogen metabolism.•600 mg/L aniline has a negative effect on the abundance of nitrogen removal genes.•Aniline biodegradation and nitrogen metabolism mechanism was elucidated. An enhanced sequencing batch reactor (SBR) system was developed to treat synthetic wastewater rich in 600 mg/L aniline. The aniline degradation efficiency was almost 100%, and the total nitrogen (TN) removal rate was more than 50%. Metagenomics technology revealed the community structure, functional genes and metabolic mechanism during the start-up of the enhanced reactor. Sequencing results showed that Proteobacteria, Bacteroidetes, Chloroflexi and Actinobacteria were dominant phylum. The proportion of degradation of aromatic compounds function increased gradually, but the proportion of nitrogen metabolism function changed little. Functional genes involved in aniline degradation including benA-xylX and dmpB/xylE were detected. The functional genes of nitrogen metabolism were involved in complete nitrification, traditional denitrification, assimilation nitrate reduction and dissimilation nitrate reduction. The functional contribution analysis and network analysis showed that the cooperation and competition of Thauera, Delftia, Diaphorobacter, Micavibrio and Azoarcus ensured the effective removal of aniline and nitrogen.