NACP/α-synuclein immunoreactivity in fibrillary components of neuronal and oligodendroglial cytoplasmic inclusions in the pontine nuclei in multiple system atrophy

• Published in: Acta neuropathologica Vol. 96; no. 5; pp. 439 - 444
• Main Authors: ARIMA, K, UEDA, K, SUNOHARA, N, ARAKAWA, K, HIRAI, S, NAKAMURA, M, TONOZUKA-UEHARA, H, KAWAI, M
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.11.1998; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Alzheimer's disease; Amyloid precursor protein; Animals; Antibodies; Atrophy; Biological and medical sciences; Degeneration; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Female; Humans; Immunoelectron microscopy; Immunohistochemistry; Immunoreactivity; Inclusion bodies; Inclusion Bodies - metabolism; Inclusion Bodies - pathology; Male; Medical sciences; Microscopy, Immunoelectron; Middle Aged; Multiple System Atrophy - metabolism; Multiple System Atrophy - pathology; Nerve Tissue Proteins - metabolism; Neurodegenerative diseases; Neurofibrils - metabolism; Neurofibrils - pathology; Neurology; Neuronal-glial interactions; Neurons - metabolism; Neurons - pathology; Oligodendroglia - metabolism; Oligodendroglia - pathology; Pons - metabolism; Pons - pathology; Pontine nuclei; Rats; Synuclein; Synucleins; Human; Immunohistochemistry; Nervous system diseases; Exploration; Cerebral disorder; Pathology; Oligodendrocyte; Central nervous system disease; Pons varolii; Multiple system atrophy; Cell inclusion; Degenerative disease; Cytoplasm; Brain (vertebrata)
• ISSN: 0001-6322; 1432-0533
• DOI: 10.1007/s004010050917

We examined neuronal cytoplasmic inclusions (NCIs) and oligodendrocytic glial cytoplasmic inclusions (GCIs) in the pontine nuclei in multiple system atrophy (MSA) using antibodies against the non-amyloid beta component of Alzheimer's disease amyloid precursor protein (NACP/alpha-synuclein). Our immunohistochemical study revealed that anti-NACP antibodies labeled both NCIs and GCIs. Immunoelectron microscopy showed that positive reaction products were localized on the 15- to 30-nm-thick filamentous components of NCIs and GCIs. The present study demonstrates that NACP is associated with cytoplasmic inclusions of MSA, and suggests a role of NACP in abnormal filament aggregation in neuronal degeneration.