Quantitative analysis of IAA in DR5::GUS transgenic arabidopsis plants

In the study of auxin transport, transgenic constructs, including DR5::GUS, are widely used for visualization of phytohormone localization. Previously we proposed a method for quantitative evaluation of the IAA content by histochemical staining for glucuronidase activity. In this work, this method w...

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Published inRussian journal of plant physiology Vol. 60; no. 3; pp. 431 - 436
Main Authors Pozhvanov, G. A, Shavarda, A. L, Medvedev, S. S
Format Journal Article
LanguageEnglish
Published Dordrecht Springer-Verlag 01.05.2013
SP MAIK Nauka/Interperiodica
Subjects
Arabidopsis thaliana
Biomedical and Life Sciences
gas chromatography
indole acetic acid
Life Sciences
mass spectrometry
Methods
Plant Physiology
Plant Sciences
quantitative analysis
transgenic plants
IAA redistribution
GUS reporter
mass-spectrometry
gas chromatography
digital image analysis
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Summary:In the study of auxin transport, transgenic constructs, including DR5::GUS, are widely used for visualization of phytohormone localization. Previously we proposed a method for quantitative evaluation of the IAA content by histochemical staining for glucuronidase activity. In this work, this method was complemented by quantitative data on the content of IAA in plants obtained by gas chromatography-mass spectrometry (GC/MS), which allowed more accurate characterization of the lateral IAA gradient arising at the Arabidopsis thaliana (L.) Heynh (ecotype Columbia 0) root gravistimulation. Applied method of IAA analysis, combining GC/MS and histochemistry, can be used for quantitatification of the other plant hormone distribution in transgenic plants with the GUS reporter.
Bibliography:http://dx.doi.org/10.1134/S1021443713020179
ObjectType-Article-2
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content type line 23
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443713020179