Pleiotropic effect of the fas5 mutation on the shoot development of Arabidopsis thaliana

• Published in: Russian journal of developmental biology Vol. 46; no. 1; pp. 10 - 18
• Main Authors: Albert, E. V, Kavai-ool, U. N, Ezhova, T. A
• Format: Journal Article
• Language: English
• Published: Moscow Springer-Verlag 2015; Pleiades Publishing
• Subjects: Animal Anatomy; apical meristems; Arabidopsis thaliana; Biomedical and Life Sciences; buds; Developmental Biology; Developmental Genetics; DNA; endopolyploidy; fasciation; gene expression; genes; Histology; leaf meristems; Life Sciences; Morphology; mutants; mutation; neoplasms; ontogeny; phenotype; pleiotropy; gene interaction; apical meristem; mutants; genetic control of shoot development
• ISSN: 1062-3604; 1608-3326
• DOI: 10.1134/S1062360415010038

The paper describes a new mutation that causes the development of multiple meristematic foci as part of shoot apical meristem, which can give rise to new stem axes or cause stem fasciation. The wus-1 mutation represses development of additional apical meristem in fas5 mutant, indicating the sequential action of the genes in the formation of the shoot apical meristem and FAS5 gene participation in spatial restriction of the WUS gene expression. Gene FAS5 performs this function independently of other negative regulators of WUS gene, namely genes CLV, as demonstrated by additive phenotype of double mutants fas5 clv2-1 and fas5 clv3-2. Besides the effect on the development of the shoot apical meristem, fas5 mutation causes a change in the shape and number of leaves, accelerates the plant transition to the reproductive stage and leads to the development of cell neoplasms on the stem (buds, stigmatic tissues, and ovule-like structures). The mutation also causes changes in apical meristems and leaf cell morphology indicating the activation in cells of DNA endoreduplication. Pleiotropic effect of the fas5 mutation on different stages of ontogeny and different organs suggests that the FAS5 gene plays a complex regulatory role at all stages of A. thaliana shoot development and affects many direct or indirect target genes.