Elucidating temperature on mixotrophic cultivation of a Chlorella vulgaris strain: Different carbon source application and enzyme activity revelation

• Published in: Bioresource technology Vol. 314; p. 123721
• Main Authors: Zhang, Zengshuai, Gao, Pengtao, Guo, Liang, Wang, Yi, She, Zonglian, Gao, Mengchun, Zhao, Yangguo, Jin, Chunji, Wang, Guangce
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2020
• Subjects: Bicarbonate; Chlorella vulgaris; CO2 capture; Enzyme activity; Mixotrophic cultivation; Bicarbonate; Enzyme activity; CO2 capture; Mixotrophic cultivation; Chlorella vulgaris
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2020.123721

[Display omitted] •Effect of temperature on mixotrophic cultivation of microalgae was investigated.•The optimum temperature was at the range of 15 °C–20 °C of Chlorella vulgaris.•The activity of Rubisco was obviously inhibited at the temperature of 30 °C.•Citrate synthase was not susceptible to the increasing temperature. With depletion of fossil fuel, microalgae is considered as a promising substitute due to high growth rate, efficient cost and high biofuels content. This study investigated the effect of temperature on mixotrophic cultivation of Chlorella vulgaris. In addition, the combination carbon source of inorganic (HCO3− or CO32−) and organic (glucose or acetate) for microalgae cultivation was evaluated to obtain the optimum carbon source for mixotrophic cultivation. The results showed that the optimum temperature of microalgae cultivation was at the range of 15–20 °C. The activity of Rubisco was obviously inhibited at the temperature of 30 °C, however, citrate synthase was not susceptible to the increasing temperature. COD removal efficiency was all higher than 64.0%. Low temperature was benefit for protein formation, and the lipid accumulation occurred at high temperature. The results provide a fresh perspective between enzyme activity and temperature variation for product accumulation of microalgae.