Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has received significant attention over the last 10 years and has been widely used for the analysis of biological samples. The technique allows the determination of elements and isotopes in biological tissues and related materia...
Saved in:
| Published in | Journal of analytical atomic spectrometry Vol. 29; no. 12; pp. 224 - 2228 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
01.01.2014
|
| Online Access | Get full text |
Cover
Loading…
| Summary: | Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has received significant attention over the last 10 years and has been widely used for the analysis of biological samples. The technique allows the determination of elements and isotopes in biological tissues and related materials with a spatial resolution typically ranging from 10 to 100 μm. When compared to other techniques usually employed to obtain bioimages, the greater advantage of LA-ICP-MS is its higher sensitivity. The literature survey over the last 10 years concerning the use of LA-ICP-MS for biological tissue analysis is reviewed in this article. Instrumentation, strategies of calibration for quantitative analysis, challenges and recent advances in this field are discussed. Applications of the isotope ratio (IR), including tracer experiments, and isotope dilution (ID), are reviewed for biological samples (briefly for proteins, in order to show the utility of LA-ICP-MS). Bioimaging methods, studies and applications for animal and plants tissues are emphasized, demonstrating the importance of bioimaging of metals and metalloids in biomedical research, bioaccumulation and bioavailability studies for ecological and toxicological risk assessment in humans, animals and plants. The usefulness of the IR associated with bioimaging for predicting geographical origin, habitat, movement of subjects, diet and lifestyle are also demonstrated.
Laser ablation inductively coupled plasma mass spectrometry has been proven to be useful in life sciences as can be observed by the increasing number of publications in this field. |
|---|---|
| Bibliography: | Guilherme Luiz Scheffler is a PhD student at the Chemistry Institute of Federal University of Rio Grande do Sul, Brazil. His research is about the use of mixed gas plasmas and different sample introduction systems into the ICP for both ICP OES and ICP-MS techniques. Valderi L. Dressler is professor at the Federal University of Santa Maria (Brazil), Department of Chemistry. He has experience in Analytical Chemistry mainly on method development for element determination using inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP OES) as well as hyphenation of ICP-MS with laser ablation (LA), electrothermal vaporization (ETV) and chromatography (LC and GC). Dirce Pozebon is professor at the Federal University of Rio Grande do Sul (Chemistry Institute, Porto Alegre, RS, Brazil). Her research interests have dealt with ICP-MS (inductively coupled plasma mass spectrometry associated with ETV (electrothermal vaporization) and LA (laser ablation), ICP OES (inductively coupled plasma optical emission spectrometry), and development of methods for trace elements determination. Matheus A. G. Nunes is a PhD student at the Chemistry Department of the Federal University of Santa Maria in Brazil. The topic of his thesis is sample preparation methods and calibration strategies for plant tissue analysis using LA-ICP-MS. |
| ISSN: | 0267-9477 1364-5544 |
| DOI: | 10.1039/c4ja00250d |