Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins

• Published in: Biomedical and environmental sciences Vol. 24; no. 4; pp. 415 - 421
• Main Authors: ZHANG, XiaoPeng, XIANG, Qian, CUI, WenMing, JIA, XuDong, LI, Ning
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2011; Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China
• Subjects: Almond skins; Animals; Bone Marrow Cells - drug effects; Chromosome aberration; Chromosome Aberrations - chemically induced; Female; Genotoxicity; Male; Mice; Micronuclei; Micronucleus Tests; Mutagenicity; Plant Extracts - chemistry; Plant Extracts - toxicity; Prunus - chemistry; Salmonella typhimurium - drug effects; Seeds - chemistry; 体内试验; 体外; 使用剂量; 杏仁; 染色体畸变试验; 评价; 遗传毒性; 鼠伤寒沙门氏菌; Almond skins; Mutagenicity; Chromosome aberration; Micronuclei; Genotoxicity
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/0895-3988.2011.04.013

Objective It aims to study potential genotoxicity of almond skins.Methods A bacterial reverse mutation assay was performed on S.typhimurium strains TA97,TA98,TA100,TA102,and TA1535 in the absence or presence of S-9 mixture at a dose range of 312.5 to 5 000 μg/plate.A micronucleus test and a mammalian bone marrow chromosome aberration tests were performed in Swiss Albino （CD-1） mice at doses of 625,1 250,and 2 500 mg/kg bw used.Results Almond skins exerted no mutagenic activity in various bacterial strains of Salmonella typhimurium in either the absence or the presence of metabolic activation at all doses tested.Various doses of almond skins did not affect the proportions of immature to total erythrocytes,the number of micronuclei in the immature erythrocytes,or the number of structural and numerical chromosomal aberrations of Swiss albino mice.Conclusion Almond skins are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and two in vivo tests-micronucleus test and mammalian bone marrow chromosome aberration test,which supports the safety of almond skins for dietary consumption.