Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides

Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.

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Bibliographic Details
Published inChemical communications (Cambridge, England) no. 12; pp. 1327 - 1329
Main Authors Jahn, Michael, Stoll, Dominik, Warren, R Antony J, Szabó, Lóránd, Singh, Pritpal, Gilbert, Harry J, Ducros, Valérie M A, Davies, Gideon J, Withers, Stephen G
Format Journal Article
LanguageEnglish
Published England 01.01.2003
Subjects
Carbohydrate Sequence
Catalysis
Cellulases - chemistry
Glycopeptides - biosynthesis
Glycopeptides - chemistry
Glycoside Hydrolases - chemistry
Glycosylation
Hydrogen-Ion Concentration
Mannans - chemistry
Mannosidases - chemistry
Models, Molecular
Molecular Sequence Data
Multienzyme Complexes - chemistry
Mutagenesis, Site-Directed
Oligosaccharides - biosynthesis
Oligosaccharides - chemistry
Time Factors
Transferases - chemistry
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Summary:Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.
ISSN:1359-7345
1364-548X
DOI:10.1039/b302380j