Gas chromatography-mass spectrometry methods for analysis of 2- and 3-hydroxylated fatty acids: Application for endotoxin measurement

Gas chromatography-mass spectrometry (GC-MS) methods for analysis of 2- and 3-hydroxy fatty acids were evaluated. These acids were analysed as methyl-trimethylsilyl or methyl-pentafluorobenzoyl derivatives in the electron impact and chemical ionization-negative ion (NICI)_modes, respectively. NICI s...

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Published inJournal of microbiological methods Vol. 17; no. 2; pp. 91 - 102
Main Authors Mielniczuk, Zbigniew, Mielniczuk, Eugenia, Larsson, Lennart
Format Journal Article
LanguageEnglish
Published Shannon Elsevier B.V 1993
Elsevier Science
Subjects
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Dust
Endotoxin
Environment
Fundamental and applied biological sciences. Psychology
GCMS
Hydroxy acid
Microbiology
Environment
Dust
GCMS
Endotoxin
Hydroxy acid
Gas chromatography
Hydroxyacid
Investigation method
Lipopolysaccharide
Detection
Fatty acids
Mass spectrometry
Quantitative analysis
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Summary:Gas chromatography-mass spectrometry (GC-MS) methods for analysis of 2- and 3-hydroxy fatty acids were evaluated. These acids were analysed as methyl-trimethylsilyl or methyl-pentafluorobenzoyl derivatives in the electron impact and chemical ionization-negative ion (NICI)_modes, respectively. NICI spectra were strongly influenced by the temperature of the ion source. Both 2- and 3-hydroxylated acids with 10–18 carbon atoms were detected in organic dust collected from a ventilation duct; differential ion monitoring was used for their separation. The described GC-MS methods can be applied for determination of air-borne endotoxins.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/0167-7012(93)90002-Y