Effect of poly (lactic acid) porous membrane prepared via phase inversion induced by water droplets on 3T3 cell behavior

• Published in: International journal of biological macromolecules Vol. 183; pp. 2205 - 2214
• Main Authors: Liu, Changjun, Qiao, Weihua, Wang, Chaorong, Wang, Han, Zhou, Yingshan, Gu, Shaojin, Xu, Weilin, Zhuang, Yan, Shi, Jiawei, Yang, Hongjun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 31.07.2021
• Subjects: 3T3 cell; Cell behavior; Phase inversion; Porous membranes; Phase inversion; 3T3 cell; Cell behavior; Porous membranes
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2021.05.197

Phase inversion induced by water droplets has garnered attention in the field of polymer science as a novel method for preparing porous membranes. This study investigates the effect of the porous structure of poly (lactic acid) (PLA) membranes prepared through phase inversion induced by water droplets at four different temperatures (25, 50, 75, and 100 °C) on the morphology and proliferation of 3T3 cells. The surface properties of the PLA porous membrane, including pore size, pore size distribution, surface roughness, surface hydrophilicity, and cytocompatibility with 3T3 cells, were evaluated. The results indicated that the synthesized PLA membrane had two surfaces with different structures. The upper surface in contact with the water droplets during preparation contained uniformly distributed micropores, whereas the bottom surface was smooth and composed of small particles in contacted with the mold. The upper surface showed high cytocompatibility with 3T3 cells, and the 3T3 cells migrated and grew within the pores at 25 °C. In contrast, the bottom surface exhibited low biocompatibility with the 3T3 cells. Our study has wide-ranging implications and will improve the fabrication and implementation of 3D cultured scaffolds with excellent cytocompatibility.