novel stress-related gene in developing pepper anthers

• Published in: Russian journal of plant physiology Vol. 56; no. 5; pp. 654 - 662
• Main Authors: Lee, Y, Chae, K, Ha, J, Lee, J. S, Jang, I. C, Jeong, S, Kim, M, Yoon, M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.09.2009; SP MAIK Nauka/Interperiodica
• Subjects: Alcohol dehydrogenase; anther development; Anthers; Arabidopsis; Biomedical and Life Sciences; Capsicum annuum; Color; Data processing; DEGs; Developmental stages; Embryogenesis; Flowers; Gene expression; Heat treatments; Life Sciences; microspore embryogenesis; Nucleotide sequence; Plant Physiology; Plant Sciences; Primers; Research Papers; Solanum tuberosum; Stress; stress; Capsicum annuum; DEGs; microspore embryogenesis; anther development
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443709050112

To study gene expression patterns and to find genes related with microspore embryogenesis during pepper (Capsicum annuum L.) anther development, mRNA expression patterns were investigated at four developmental stages distinguished according to the size of flower bud, the color of anthers, and the cytological feature of microspores. Through GeneFishing using 120 random primers, 81 genes were found to be differentially expressed as anthers develop. We directly sequenced seven of them, which were either up- or down-regulated at stage 2, since microspores at stage 2 are known to be responsive to the induction signals for microspore embryogenesis. Nucleotide sequence analysis of the isolated differentially expressed genes (DEGs) and the comparison of these sequences with the GenBank data indicate that DEG13 is a novel gene, which is highly homologous to a stress-related gene of potato, POACT88 ([almost equal to]91%) and to alcohol dehydrogenase gene of Arabidopsis ([almost equal to]70%), whose expression is also tightly related to stresses. In vitro data also showed that DEG13 was more abundantly expressed in heat-treated microspores than in untreated microspores. Here, we report developmental stage-specific gene expression patterns during anther development and a novel stress-related gene, DEG13, which may be involved in microspore embryogenesis in response to heat treatment.