Conventional optimization and characterization of microbial dextran using treated sugarcane molasses

• Published in: International journal of biological macromolecules Vol. 220; pp. 775 - 787
• Main Authors: Rahman, Sameeha Syed Abdul, Pasupathi, Saroja, Karuppiah, Sugumaran
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2022
• Subjects: Dextran; Structural characterization; Sugarcane molasses; Dextran; Structural characterization; Sugarcane molasses
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.08.094

This study focuses the comparison on yield of microbial dextran using treated sugarcane molasses (SCM) as a feed stock from different treatment methods. The suitable method for treatment of SCM was identified on the basis of microbial dextran production. The different factors namely the concentrations of total sugars, nitrogen sources, inoculum size, shaking speed, initial medium pH, and phosphate sources influencing the production of microbial dextran were studied. The maximum yield of dextran was obtained to be 17.18 ± 0.08 g L−1 using the conventional optimization. The structural analysis of produced dextran from SCM with various treatment techniques was compared using Fourier-transform infra-red analysis and nuclear magnetic resonance spectroscopy. Later, the rheological behavior of produced microbial dextran was examined and found to be a non-Newtonian. To the best of our knowledge, the comparison on the production of microbial dextran through fermentation using SCM with various treatment strategies has not been performed yet.