The Active Species of "CO2" Utilized by Ribulose Diphosphate Carboxylase

The active species of "CO 2 ", i.e. CO 2 or HCO 3 - , utilized in the ribulose diphosphate carboxylase reaction was determined with the use of homogeneous preparations of the spinach enzyme. Employing an assay system predicated upon the observation that the hydration of CO 2 requires more...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 244; no. 4; pp. 1081 - 1083
Main Authors Cooper, T G, Filmer, D, Wishnick, M, Lane, M D
Format Journal Article
LanguageEnglish
Published American Society for Biochemistry and Molecular Biology 01.02.1969
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Summary:The active species of "CO 2 ", i.e. CO 2 or HCO 3 - , utilized in the ribulose diphosphate carboxylase reaction was determined with the use of homogeneous preparations of the spinach enzyme. Employing an assay system predicated upon the observation that the hydration of CO 2 requires more than 60 sec to reach equilibrium at temperatures below 15°, data were obtained which are quite compatible with those expected if CO 2 is the active species.
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(18)91899-5