Micropropagation of Cascade Huckleberry, Mountain Huckleberry, and Oval-leaf Bilberry Using Woody Plant Medium and Murashige and Skoog Medium Formulations

Two concentrations of two in vitro media formulations were evaluated for their effects on survival, shoot growth, and percentage rooting of cascade huckleberry (Vaccinium deliciosum), mountain huckleberry (V. membranaceum), and oval-leaf bilberry (V. ovalifolium). Two-node stem sections from establi...

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Bibliographic Details
Published inHortTechnology (Alexandria, Va.) Vol. 17; no. 3; pp. 279 - 284
Main Authors Barney, D.L, Lopez, O.A, King, E
Format Journal Article
LanguageEnglish
Published 2007
Subjects
culture media
dry matter accumulation
in vitro regeneration
micropropagation
mortality
nutrient solutions
photoperiod
rooting
species differences
Vaccinium
Vaccinium deliciosum
Vaccinium membranaceum
Vaccinium ovalifolium
viability
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Summary:Two concentrations of two in vitro media formulations were evaluated for their effects on survival, shoot growth, and percentage rooting of cascade huckleberry (Vaccinium deliciosum), mountain huckleberry (V. membranaceum), and oval-leaf bilberry (V. ovalifolium). Two-node stem sections from established microshoots were cultured on full- or half-strength modified Murashige and Skoog medium (FSMS and HSMS) or full- or half-strength modified woody plant medium (FSWPM and HSWPM) unamended with plant growth regulators. Cultures were maintained at 21 degrees C with a 16-hour photoperiod for 98 days. Survival on FSMS was reduced by approximately equal to 44% for cascade huckleberry, 63% for mountain huckleberry, and 18% for oval-leaf bilberry compared with average survival on HSMS, HSWPM, and FSWPM. Explants on FSMS also produced new shoot growth having the lowest dry weights, fewest shoots, and shortest shoots of the four media. Explant rooting percentages were also least on FSMS. For cascade huckleberry and oval-leaf bilberry, HSMS, HSWPM, and FSWPM all appeared suitable for general culture. For mountain huckleberry, both woody plant medium formulations produced greater microshoot dry weights, average shoot lengths, and explant rooting percentages compared with HSMS. These results are the first published on micropropagation for cascade huckleberry and oval-leaf bilberry, and provide starting protocols for commercial propagation and further research on micropropagation of these species.
ISSN:1063-0198
1943-7714
DOI:10.21273/horttech.17.3.279