A new application of in situ hybridization: detection of numerical and structural chromosome aberrations with a combination centromeric-telomeric DNA probe

Fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used high-resolution, single-color fluorescent in situ hybridization with a combination centromeric-telomeric DNA probe, specific for chromosome 1, to investigate the feasibility of sim...

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Bibliographic Details
Published inCytogenetics and cell genetics Vol. 48; no. 3; p. 188
Main Authors van Dekken, H, Bauman, J G
Format Journal Article
LanguageEnglish
Published Switzerland 1988
Subjects
Centromere - ultrastructure
Chromosome Aberrations
Chromosomes - ultrastructure
Chromosomes, Human, Pair 1
DNA Probes
Humans
Microscopy, Fluorescence
Nucleic Acid Hybridization
Tumor Cells, Cultured
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Summary:Fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used high-resolution, single-color fluorescent in situ hybridization with a combination centromeric-telomeric DNA probe, specific for chromosome 1, to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. The K562 leukemia cell line served as a model.
ISSN:0301-0171
DOI:10.1159/000132622