Comparative analysis of the effectiveness of STR and SNP markers for intraspecific and interspecific differentiation of the genus Ovis

• Published in: Russian journal of genetics Vol. 52; no. 1; pp. 79 - 84
• Main Authors: Deniskova, T. E., Sermyagin, A. A., Bagirov, V. A., Okhlopkov, I. M., Gladyr, E. A., Ivanov, R. V., Brem, G., Zinovieva, N. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2016
• Subjects: Animal Genetics; Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; Human Genetics; Microbial Genetics and Genomics; Ovis; microsatellites; local sheep breeds; whole genome screening; non-model organisms
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795416010026

A comparative study of the informativeness of SNP and STR markers for interspecific and intraspecific differentiation of the two species of the genus Ovis, snow sheep (O. nivicola) and domestic sheep (O. aries), was conducted. Eleven STR loci combined into two multiplex panels were examined. SNP analysis was performed with the DNA microarray OvineSNP50K BeadChip featuring 54241 SNPs. The possibility of clear differentiation of the studied Ovis species with both types of genetic markers was demonstrated. The advantages of SNP markers for intraspecific differentiation of the O. aries breeds and O. nivicola geographical groups were revealed. The areas of application of the studied types of DNA markers are discussed.