Enhanced antilipopolysaccharide (LPS) induced changes in macrophage functions by Rubia cordifolia (RC) embedded with Au nanoparticles

• Published in: Free radical biology & medicine Vol. 65; pp. 217 - 223
• Main Authors: Singh, Ashwani Kumar, Tripathi, Yamini B, Pandey, Nidhi, Singh, D.P, Tripathi, Deepshikha, Srivastava, O.N
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2013
• Subjects: Animals; Anti-Inflammatory Agents - administration & dosage; Cells, Cultured; Chromatography, High Pressure Liquid; gold; Gold - administration & dosage; inhibitory concentration 50; lemons; lipopolysaccharides; Lipopolysaccharides - toxicity; macrophages; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - immunology; Metal Nanoparticles - chemistry; Nanoconjugates - chemistry; nanogold; nitric oxide; Plant Extracts - administration & dosage; Rats; Rubia - chemistry; Rubia cordifolia; transmission electron microscopy; ultraviolet-visible spectroscopy; X-ray diffraction; Herbs; NO; Rubia cordifolia-embedded gold nanoparticles; Gold nanoparticles; Rubia cordifolia; RC-Au (NPs); SAED; energy dispersive X-ray analysis; Au (NPs); LPS; RC; selected area electron diffraction pattern; EDAX; Nitric oxide; lipopolysaccharide; Anti-inflammatory
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2013.06.006

In this paper, we have shown that gold nanoparticles (Au (NPs)) embedded in Rubia cordifolia (RC) matrix (RC-Au (NPs)) exhibit a high therapeutic value relating to its anti-inflammatory characteristics. It was prepared by utilizing the reducing properties of RC to convert HAuCl₄ into Au (NPs). In order to compare its effectiveness, with respect to Au (NPs), the latter was synthesized separately by reducing HAuCl₄ with lemon extract. These Au (NPs) along with RC-Au (NPs) were characterized by X-ray diffractometry (XRD), transmission electron microscopy (TEM), and UV-visible spectroscopy. The enhancement in anti-inflammatory characteristics was assessed as its inhibitory potential for lipopolysaccharide (LPS)-induced nitric oxide (NO) release, by rat peritoneal macrophages. The RC-Au (NPs) significantly enhanced its potential to inhibit NO release, which was reported in terms of inhibitory concentration for 50% inhibition (IC₅₀=11.98ng/ml), as compared to either RC extract (IC₅₀=47 × 10³ng/ml) or to Au (NPs) (IC₅₀=587.50ng/ml).