RFLP and microsatellite mapping of gene for soybean mosaic virus resistance

• Published in: Phytopathology Vol. 84; no. 1; pp. 60 - 64
• Main Authors: Yu, Y.G, Sahgai, M.A, Saghai Maroof, M.A, Buss, G.R, Maughan, P.J, Tolin, S.A
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.01.1994
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Fundamental and applied biological sciences. Psychology; GENE; Generalities. Genetics. Plant material; GENES; GENETICA; Genetics and breeding of economic plants; GENETIQUE; GLYCINE MAX; LIGNEE CONSANGUINE; LINEAS CONSANGUINEAS; MARCADORES GENETICOS; MARQUEUR GENETIQUE; Molecular genetics; POLIMORFISMO; POLYMORPHISME; POTYVIRUS DEL MOSAICO DE LA SOJA; POTYVIRUS MOSAIQUE SOJA; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; VARIEDADES; VARIETE; Plant pathogen; Genetic marker; Microorganism resistance; Gene; Dicotyledones; Angiospermae; Genetics; Molecular probe; Potyvirus; Nucleic acid; Genetic mapping; Soybean mosaic virus; Glycine max; Oil plant(vegetal); Virus; Grain legume; Sensitivity resistance; Restriction fragment length polymorphism; Leguminosae; Plant pathogen resistance; DNA; Plant origin; Spermatophyta; Molecular biology; Genome
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/phyto-84-60

Restriction fragment length polymorphisms (RFLPs) and Microsatellites or simple sequence repeats (SSRs) were used as genetic markers to identify the chromosomal location of Rsv, a gene conferring resistance to soybean mosaic virus (SMV). An population was constructed from a cross between soybean line PI 96983 as the resistant parent and cultivar Lee 68 as the susceptible parent. Twenty-five RFLP and three SSR loci, polymorphic between the parental lines, were analyzed in 107 F2 individuals. Genotypes of Rsv were determined by inoculating F2:3 progeny with the G1 strain of SMV. Data also were collected for an additional soybean gene (w1/W1), which controls anthocyanin pigmentation in hypocotyls and flowers. Analyses of the data revealed that the SSR marker, SM176 (a soybean heat shock protein gene), and two RFLP markers, pA186 and pK644a, are closely linked to Rsv, with distances of 0.5, 1.5, and 2.1 centiMorgans, respectively. The close links between Rsv and the three markers were confirmed by marker analysis of three Williams near-isogenic lines (NILs) that carry Rsv alleles from PI 96983 and Marshall. Marker analysis also indicated that the SMV resistance gene in Buffalo is probably at the Rsv locus