Immunomodulatory and antibacterial activities of extracts from Rutaceae species

Rutaceae is a taxon with species very well distributed in Brazilian semi-arid area, commonly used in folk medicine. Species from this genus have diverse biological activity described in literature. In this work, immunomodulatory and bactericidal activity are described for chloroform and ethyl acetat...

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Published inRevista brasileira de farmacognosia Vol. 20; no. 4; pp. 502 - 505
Main Authors Costa, José Fernando Oliveira, Juiz, Paulo, São Pedro, André, David, Juceni P. de L., David, Jorge M., Giulietti, Ana Maria, França, Flávio, Santos, Ricardo Ribeiro dos, Soares, Milena B. P.
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Farmacognosia 01.09.2010
Subjects
PHARMACOLOGY & PHARMACY
antibacterial activity
Atividade imunomoduladora
atividade antibacteriana
Immunomodulatory activity
Rutaceae
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Summary:Rutaceae is a taxon with species very well distributed in Brazilian semi-arid area, commonly used in folk medicine. Species from this genus have diverse biological activity described in literature. In this work, immunomodulatory and bactericidal activity are described for chloroform and ethyl acetate extracts of three of them (Esenbeckia grandiflora Mart., Pilocarpus spicatus A.St.-Hil. and Galipea simplicifolia Schult.). Initially all the samples had their cytotoxicity evaluated, aiming to determine the LC50. The immunomodulatory potential was evaluated in cultures of murine splenocytes stimulated or not with concanavalin A and in a mixed lymphocyte reaction (MLR) using splenocytes from BALB/c (H-2d) mice immunized with splenocytes from C57Bl/6 (H-2b) mice. Four samples had higher values of lymphoproliferation inhibition in concanavalin A-stimulated cultures and were evaluated in MLR. The antibacterial activity of extracts was also evaluated and the minimal inhibitory concentrations (MIC) for two active samples were 1.0 and 5.0 mg/ml for extracts from Esenbeckia grandiflora Mart. and Galipea simplicifolia Schult., respectively. Thus, our results reinforce data of literature relating biological activity for many species of the Rutaceae family and encourage studies with these species aiming to discover active compounds, candidates to new medicines.
ISSN:0102-695X
1981-528X
DOI:10.1590/S0102-695X2010000400007