Application of Fluorescent Antibody Methods in the Analysis of Plaque Samples

Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein lab...

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Bibliographic Details
Published inJournal of dental research Vol. 55; no. 1_suppl; pp. 80 - 86
Main Authors Thomson, L. A., Little, W., Hageage, G. J.
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.01.1976
Subjects
Animals
Dental Plaque - microbiology
Dentistry
Fluorescent Antibody Technique - methods
Humans
Rhodamines
Staining and Labeling
Streptococcus - isolation & purification
Streptococcus mutans - classification
Streptococcus mutans - immunology
Streptococcus mutans - isolation & purification
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Summary:Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein label and c-, e-rhodamine label conjugates indicate that serotype c is the most common. This is in agreement with many reports by other investigators. Cross-reactions with many S mutans conjugates occurred with organisms resembling Lancefield groups C and G streptococci.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-0345
1544-0591
DOI:10.1177/002203457605500126011