High titer (>200 g/L) lactic acid production from undetoxified pretreated corn stover

• Published in: Bioresource technology Vol. 388; p. 129729
• Main Authors: Zhang, Yuwei, Xu, Zhaoxian, Lu, Minrui, Ma, Xingwang, Chen, Sitong, Wang, Yanchen, Shen, Wenyuan, Li, Pingping, Jin, Mingjie
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2023
• Subjects: Corn stover; Lactic acid fermentation; Lactobacillus pentosus; Lignocellulosic biorefinery; Xylose fermentation; Xylose fermentation; Lactobacillus pentosus; Lactic acid fermentation; Corn stover; Lignocellulosic biorefinery
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129729

[Display omitted] •Higher than 200 g/L lactic acid was obtained from DLC-pretreated corn stover.•Cellulosic lactic acid fermentation was realized at 40% solid loading.•Cellulosic lactic acid fermentation was significantly improved by SSCF.•L. pentosus LB-1 can co-utilize glucose, xylose, arabinose, galactose and mannose.•No detoxify units are required for DLC pretreatment based biorefinery system. Lignocellulosic biomass is a reliable feedstock for lactic acid fermentation, low product titers hamper the scale production of cellulosic lactic acid. In this study, a Densifying Lignocellulosic biomass with Chemicals (sulfuric acid) pretreatment based cellulosic lactic acid biorefinery system was developed and demonstrated from multi-dimensions of producing bacteria, fermentation modes, corn stover solid loadings, fermentation vessels, and product purification. Results suggested that several lactic acid bacteria exhibited high fermentation activity in high solid loading corn stover hydrolysates. Remarkably, simultaneous saccharification co-fermentation performed in 100-mL flasks enabled 210.1 g/L lactic acid from 40% solid loading corn stover hydrolysate. When simultaneous saccharification co-fermentation was performed in 3-L bioreactors, 157.4 g/L lactic acid was obtained from 35% solid loading corn stover hydrolysate. These obtained lactic acid titers are the highest reports until now when lignocellulosic biomasses are used as substrates, making it efficient for scale production of cellulosic lactic acid.