Ca2+ Imaging in Perfused Adrenal Medullae

The Ca2+ imaging method was developed to explore changes in excitability in adrenal medullary (AM) cells in a large field in response to synaptic input and chemicals. The adrenal medullae of rats and guinea pigs were retrogradely loaded with Ca2+ indicator through the adrenal vein. Nerve fibers rema...

Full description

Saved in:
Bibliographic Details
Published inJournal of UOEH Vol. 34; no. 2; pp. 163 - 173
Main Authors WARASHINA, Akira, INOUE, Masumi
Format Journal Article
LanguageEnglish
Japanese
Published Japan The University of Occupational and Environmental Health, Japan 01.06.2012
Subjects
adrenal medulla
Adrenal Medulla - drug effects
Adrenal Medulla - innervation
amperometry
Animals
Ca2+ imaging
Calcium
catecholamine
Electric Stimulation
gamma-Aminobutyric Acid - pharmacology
Guinea Pigs
Male
Perfusion
Rats
Rats, Wistar
secretion
Online AccessGet full text

Cover

More Information
Summary:The Ca2+ imaging method was developed to explore changes in excitability in adrenal medullary (AM) cells in a large field in response to synaptic input and chemicals. The adrenal medullae of rats and guinea pigs were retrogradely loaded with Ca2+ indicator through the adrenal vein. Nerve fibers remaining in the adrenal gland were electrically stimulated to induce postsynaptic responses in AM cells, and chemicals were applied to the cells by adding to the perfusate. With this method, γ-aminobutyric acid (GABA) was shown to increase the Ca2+ signal in almost all and 40% AM cells in guinea pigs and rats, respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0387-821X
2187-2864
DOI:10.7888/juoeh.34.163