Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene

The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPECFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamella...

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Published inBiochemistry and molecular biology international Vol. 47; no. 2; p. 337
Main Authors Serafino, A L, Novelli, G, Di Sario, S, Colosimo, A, Amicucci, P, Sangiuolo, F, Mossa, G, Dallapiccola, B
Format Journal Article
LanguageEnglish
Published England 01.02.1999
Subjects
Cell Line
Cystic Fibrosis Transmembrane Conductance Regulator - genetics
Cystic Fibrosis Transmembrane Conductance Regulator - metabolism
DNA Adducts - chemistry
Gene Transfer Techniques
Genetic Therapy - methods
Humans
Liposomes - chemistry
Microscopy, Confocal
Microscopy, Electron
Transfection - methods
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Summary:The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPECFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.
ISSN:1039-9712
DOI:10.1080/15216549900201363