In vitro regulation of IL-6 and TGF-ß by mesenchymal stem cells in systemic lupus erythematosus patients

• Published in: Medicinski glasnik Vol. 17; no. 2; pp. 408 - 413
• Main Authors: Masyithah Darlan, Dewi, Munir, Delfitri, Karmila Jusuf, Nelva, Putra, Agung, Ikhsan, Riyadh, Alif, Iffan
• Format: Journal Article
• Language: English
• Published: Medical Association of Zenica-Doboj Canton 01.08.2020
• Subjects: autoimmune disease; cytokine dysregulation; immunoregulation; inflammatory disorder
• ISSN: 1840-0132; 1840-0132; 1840-2445
• DOI: 10.17392/1186-20

Aim To analyse the ability of mesenchymal stem cells (MSCs) to regulate interleukin 6 (IL-6) and transforming growth factor (TGF-β) expression in vitro under co-culture conditions in human systemic lupus erythematosus (SLE). Method This study used a post-test group design that used peripheral blood mononuclear cells (PBMCs) from SLE patients at Kariadi Hospital, Semarang, Indonesia, and MSCs from a human umbilical cord. The cells were divided into two groups. The control group of PBMCs was treated with a standard medium, and the treatment group was co-cultured with the MSCs at a 1:40 ratio. Following 24 h incubation, the levels of IL-6 and TGF-β released in the culture medium were measured using a specific ELISA assay. Results This study showed a significant decrease in IL-6 level (p< 0.05) and a significant increase in TGF-β level (p<0.001) following 24 h of co-culture incubation of human SLE PBMCs cells and MSCs. Conclusion The PBMCs-to-MSCs ratio of 1:40 can regulate the IL-6 and TGF-β levels in human SLE PBMCs.