Molecular Differentiation of Nontuberculous Mycobacterium Isolated from Different Animals

To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium...

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Published inApplied Mechanics and Materials Vol. 421; no. Information Technology for Manufacturing Systems IV; pp. 300 - 303
Main Authors Qian, Ai Dong, Qi, Hua Rui, Jiang, Xiu Yun, Wang, Chun Feng, Wang, Chun Fang, Chen, Fei Fei, Ma, Hong Xia
Format Journal Article
LanguageEnglish
Published Zurich Trans Tech Publications Ltd 11.09.2013
Subjects
Mycobacterium
Mycobacterium fortuitum
hsp65 Gene
Molecular Differentiation
16SrDNA
Nontuberculous Mycobacterium
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Abstract To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of 16SrDNA and the 65-kDa heat-shock protein gene (hsp65 gene). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals are an important environmental source of mycobacteria and the combined application of 16SrDNA and hsp65 sequencing was more reliable to accurately identify mycobacteria present in animals.
AbstractList To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of 16SrDNA and the 65-kDa heat-shock protein gene (hsp65 gene). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals are an important environmental source of mycobacteria and the combined application of 16SrDNA and hsp65 sequencing was more reliable to accurately identify mycobacteria present in animals.
To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of 16SrDNA and the 65-kDa heat-shock protein gene ( hsp65 gene ). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals are an important environmental source of mycobacteria and the combined application of 16SrDNA and hsp65 sequencing was more reliable to accurately identify mycobacteria present in animals.
Author Wang, Chun Feng
Jiang, Xiu Yun
Ma, Hong Xia
Qian, Ai Dong
Wang, Chun Fang
Qi, Hua Rui
Chen, Fei Fei
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Nontuberculous Mycobacterium
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Mycobacterium fortuitum
Title Molecular Differentiation of Nontuberculous Mycobacterium Isolated from Different Animals
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