Molecular Differentiation of Nontuberculous Mycobacterium Isolated from Different Animals

• Published in: Applied Mechanics and Materials Vol. 421; no. Information Technology for Manufacturing Systems IV; pp. 300 - 303
• Main Authors: Qian, Ai Dong, Qi, Hua Rui, Jiang, Xiu Yun, Wang, Chun Feng, Wang, Chun Fang, Chen, Fei Fei, Ma, Hong Xia
• Format: Journal Article
• Language: English
• Published: Zurich Trans Tech Publications Ltd 11.09.2013
• Subjects: Mycobacterium; Mycobacterium fortuitum; hsp65 Gene; Molecular Differentiation; 16SrDNA; Nontuberculous Mycobacterium
• ISBN: 3037858788; 9783037858783
• ISSN: 1660-9336; 1662-7482; 1662-7482
• DOI: 10.4028/www.scientific.net/AMM.421.300

To investigate the occurrence and species diversity of mycobacteria in different animals, lymphonodi mandibulares and lymphonodi mesenterici samples were collected from slaughter house of swine and cattle. Mycobacteria in each lymphonodi sample were isolated by decontamination using cetylpyridinium chloride (CPC) and cultivation on Lowenstein-Jensen medium and Middlebrook 7H9 agar, and then identified by nacterial smear, sequencing of 16SrDNA and the 65-kDa heat-shock protein gene (hsp65 gene). The most frequently isolated species was Mycobacterium fortuitum. The result demonstrated that animals are an important environmental source of mycobacteria and the combined application of 16SrDNA and hsp65 sequencing was more reliable to accurately identify mycobacteria present in animals.