In silico xanthine oxidase inhibitory activities of alkaloids isolated from Alphonsea sp
•Alphonsea sp. is a plant with many traditional uses.•Alkaloids kinabaline, atherospermidine, cyathocaline, and N-methylouregidione were isolated from alphonsea sp.•Docking studies revealed that all compounds showed interactions with amino acids through hydrogen and hydrophobic bonds. Xanthine oxida...
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Published in | South African journal of botany Vol. 147; pp. 820 - 825 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.07.2022
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Subjects | |
Online Access | Get full text |
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Summary: | •Alphonsea sp. is a plant with many traditional uses.•Alkaloids kinabaline, atherospermidine, cyathocaline, and N-methylouregidione were isolated from alphonsea sp.•Docking studies revealed that all compounds showed interactions with amino acids through hydrogen and hydrophobic bonds.
Xanthine oxidase is an enzyme responsible for the build-up of excess uric acid that causes gout, one of the most common inflammatory arthritis found in Malaysia. The present study investigate in silico xanthine oxidase inhibitory activities of alkaloids isolated from Alphonsea cylindrica and Alphonsea elliptica. Serial column chromatography led to the isolation of compounds which were characterized as kinabaline, atherospermidine, cyathocaline, and N-methylouregidione on the basis of various spectroscopic techniques. Binding orientation and binding energy of all compounds were predicted via docking studies. Docking simulations revealed that kinabaline has the lowest binding energy at 6.9040 kcal/mol. All compounds revealed at least one hydrogen bond to key amino acids. Docking study showed that atherospermidine and cyathocaline bind to active sites which suggest they are competitive inhibitors while the other two compounds are non-competitive inhibitors. The findings of this study reveal the potential of Alphonsea sp. as remedy for gout. |
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ISSN: | 0254-6299 1727-9321 |
DOI: | 10.1016/j.sajb.2022.03.024 |