Presence of Human Herpesvirus 6B in the Pancreas of Subjects With and Without Type 1 Diabetes

• Published in: Pancreas Vol. 46; no. 10; p. 1341
• Main Authors: Ericsson, Maja, Skog, Oskar
• Format: Journal Article
• Language: English
• Published: United States 01.11.2017
• Subjects: Adult; Aged; Cadaver; Diabetes Mellitus, Type 1 - complications; DNA, Viral - genetics; Female; Herpesvirus 6, Human - genetics; Herpesvirus 6, Human - metabolism; Herpesvirus 6, Human - physiology; Humans; Immunohistochemistry; Islets of Langerhans - virology; Male; Middle Aged; Pancreas - virology; Pancreas, Exocrine - virology; Polymerase Chain Reaction; Roseolovirus Infections - complications; Roseolovirus Infections - virology; Tissue Donors; Viral Proteins - metabolism; Young Adult
• ISSN: 1536-4828
• DOI: 10.1097/MPA.0000000000000927

The aims of this study were to investigate the presence of human herpesvirus 6 (HHV6) A and B in human pancreata and to search for signs of active infection in this organ of subjects with and without type 1 diabetes (T1D). Pancreata from brain-dead organ donors with and without T1D were examined for the presence of HHV6 genomic sequences by polymerase chain reaction (PCR), transcripts by reverse transcriptase-PCR, and protein by immunohistochemistry. Quantitative PCR of isolated pancreatic islets and exocrine cell clusters was used to determine the intrapancreatic location of HHV6 DNA. Human herpesvirus 6B genomic sequences were present in 1 of 2 donors who died of acute-onset T1D, 4 of 6 donors with long-standing T1D, and 9 of 12 nondiabetic donors. Higher copy numbers of HHV6B DNA were present in isolated islets than in exocrine tissue from the same donors. No signs of active HHV6 transcription were found. Human herpesvirus 6A was not present in any tested pancreas. The herein presented data demonstrate, for the first time, the presence of a latent HHV6B infection in the pancreas and islets of Langerhans. Whether this virus can contribute to disease in the pancreas remains to be determined.