Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells

• Published in: Acta pharmacologica Sinica Vol. 29; no. 9; pp. 1006 - 1012
• Main Authors: Choi, Young-whan, Kim, Kyeok, Jo, Ji-yeong, Kim, Hyo-lim, Lee, You-jin, Shin, Woo-jung, Sacket, Santosh J, Han, Mijin, Im, Dong-soon
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2008; Nature Publishing Group
• Subjects: 6＇-二甲基; Animals; Biomedical and Life Sciences; Biomedicine; Calcium - metabolism; Cell Line, Tumor; Cyclooctanes - pharmacology; Fruit - chemistry; Glioma - physiopathology; GTP-Binding Proteins - metabolism; Humans; Immunology; Indicators and Reagents; Internal Medicine; Lignans - pharmacology; Medical Microbiology; Membrane Potentials - drug effects; original-article; PC12 Cells; Pharmacology/Toxicology; Polycyclic Compounds - pharmacology; Rats; Schisandra; Schisandra - chemistry; Type C Phospholipases - metabolism; Vaccine; 五味子; 五味子素C; 木脂体; 胶质瘤; 膜蛋白质; wuweizisu C; glioma; lignan; dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybiphenyl-2,2′-dicarboxylate; membrane potential
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1111/j.1745-7254.2008.00838.x

Aim： To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells. Methods： The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells. Results： Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl- 4,4＇-dimethoxy-5,6,5＇,6＇-dimethylene dioxybiphenyl-2,2＇-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of Gi/o proteins, phospholipase C, and extracellular Na^＋ on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca^2＋ [Ca^2＋]i concentration, but decreased the ATP-induced Ca^2＋ increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC 12 neuronal cells. Conclusion： Our results suggest that the decrease in the membrane potential and the modulation of [Ca^2＋]; concentration by wuweizisu C could be important action mechanisms ofwuweizisu C.