Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells
Aim: To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells. Methods: The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded...
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Published in | Acta pharmacologica Sinica Vol. 29; no. 9; pp. 1006 - 1012 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.09.2008
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Aim: To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells. Methods: The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells. Results: Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl- 4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of Gi/o proteins, phospholipase C, and extracellular Na^+ on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca^2+ [Ca^2+]i concentration, but decreased the ATP-induced Ca^2+ increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC 12 neuronal cells. Conclusion: Our results suggest that the decrease in the membrane potential and the modulation of [Ca^2+]; concentration by wuweizisu C could be important action mechanisms ofwuweizisu C. |
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Bibliography: | wuweizisu C dimethyl- 4,4'-dimetboxy-5,6,5 ',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate lignan Schisandra chinensis membrane potential glioma R96 Schisandra chinensis; lignan; wuweizisu C; membrane potential; glioma; dimethyl- 4,4'-dimetboxy-5,6,5 ',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate 31-1347/R ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1671-4083 1745-7254 |
DOI: | 10.1111/j.1745-7254.2008.00838.x |