Infection with Micromonospora strain SB3 promotes in vitro growth of Lolium multiflorum plantlets

• Published in: Plant cell, tissue and organ culture Vol. 134; no. 3; pp. 445 - 455
• Main Authors: Della Mónica, I. F., Novas, M. V., Iannone, L. J., Querejeta, G., Scervino, J. M., Pitta-Alvarez, S. I., Regalado, J. J.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2018; Springer Nature B.V
• Subjects: Acclimatization; Agronomy; Animal husbandry; Biomass; Biomedical and Life Sciences; Endophytes; Grasses; Grasslands; Inoculation; Life Sciences; Lolium multiflorum; Micromonospora; Microorganisms; Micropropagation; Original Article; Plant biomass; Plant breeding; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Plants (botany); Actinobacteria; Micropropagation; Grass; Bacterization
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-018-1434-5

Cattle breeding is an important economical activity in Argentina, highly dependent on grass production. In the last decades, grasslands zones were reduced and confined to less productive lands due to the advance of agronomical cultures. Therefore, it is important to develop new strategies to improve forage production. New eco-friendly trends in plant growth promotion include the use of microbial endophytes, but the in vitro studies of plant-bioinoculant interactions is limited by the scarce current technological development. In this work, we use a micropropagation protocol for Lolium multiflorum , developed in a previous work, to study the effect of bacterization with actinobacterial endophytes, isolated from Argentine native grasses, on the growth of L. multiflorum in vitro plantlets. To achieve this objective, L. multiflorum plantlets were inoculated with three Micromonospora strains (SB3, TW2.1 and TW2.2). The results obtained showed that the effect of actinobacterial inoculation depends on the Micromonospora strain used. The inoculation with SB3 promoted plant growth, increasing plant biomass, root length and the rate of plantlets ready to be acclimatized after 4 weeks of in vitro culture. Strain TW2.1 did not show, statistically, differences compared to control treatments, while TW2.2 inhibited plant growth, decreasing plant biomass, root length and the rate of plants ready to acclimatize. Our results showed that Micromonospora strain SB3 could be a good candidate to use in breeding programs for L. multiflorum and other grasses to increase their yield.