Mated: a putative peptide transporter of Schizophyllum commune expressed in dikaryons

• Published in: Current genetics Vol. 36; no. 3; pp. 159 - 164
• Main Authors: Lengeler, K.B, Kothe, E
• Format: Journal Article
• Language: English
• Published: United States 01.09.1999
• Subjects: active transport; aerial mycelium; Amino Acid Sequence; amino acid sequences; Base Sequence; binding proteins; Blotting, Northern; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - physiology; Cloning, Molecular; complementary DNA; genbank/af125094; Gene Deletion; genes; Genes, Fungal - genetics; Genes, Mating Type, Fungal; Genetic Complementation Test; growth; introns; mating behavior; Membrane Transport Proteins; Molecular Sequence Data; mtd1 gene; mutants; mycelium; nucleotide sequences; open reading frames; Open Reading Frames - genetics; peptides; Phenotype; promoter regions; Protozoan Proteins; RNA - analysis; Schizophyllum - cytology; Schizophyllum - genetics; Schizophyllum commune; Sequence Analysis, DNA; Sequence Homology, Amino Acid; sexual reproduction
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s002940050486

A gene that exhibits sexually induced transcript accumulation, mtd1, was identified physically linked to a locus that is also under control of the mating-type genes. Genomic and cDNA sequence analyses were performed to determine a large open reading frame which starts with the amino-acid sequence M-A-T-E-D. The ORF showed similarities to a novel family of peptide transporters with at least 12 putative transmembrane structures. A gene disruption resulted in a growth-phenotype with slow aerial mycelium formation that could be complemented with peptone or uracil added to the growth medium.